Biocydic Effect of the Spark Discharge Non-coherent Impulse Radiation in Experiments in vitro and in vivo
The aim of work is investigation of the spark discharge plasma non-coherent impulse radiation (NCIR) use possibility as a factor capable of the microorganism growth and reproduction suppression in experiments in vitro and in vivo. The tasks of the bacteriostatic, bactericidal and fungicidal effect study, as well as the free-radical process level assessment in experiments in vitro and in vivo after the spark discharge plasma NCIR effect, were solving.
Materials and Methods. A non-coherent impulse radiation in optical range of 180—800 nm was generated in a process of a highly energetic spark discharge initiation with a use of experimental device, created on a base of the ARSRIEPh (Sarov).
The microorganism cultures S. epidermidis, strain 51-1; E. coli, strain 18M and C. albicans, strain 601 from the NNSMA microbiology and immunology chair museum are used in work. The bacterial suspensions were irradiated in modes of 5 to 600s, there was inoculation of the corresponding selective media after treatment. The intact microorganism suspension was used as a control. An assessment of the free-radical reaction level after a NCIR treatment was made in the microorganism suspension with a method of induced chemiluminescence (Fe3+ and H2O2) at a biochemiluminometer BChL-6.
An assessment of fungicidal effect and free-radical homeostasis of animals after a non-coherent impulse radiation effect in experiments in vivo is made on 72 laboratory rat-males of the Wistar line with a mass of 200±10 g by 12 samples in each series. All manipulations with animals were made with a use of ether anesthesia. The animals were divided into 6 series: the intact ones, effect of 100 s, effect of 300 s, contaminated C. albicans and effect of 100 s, contaminated C. albicans and effect of 300 s. The animals were infected perorally with a C. albicans suspension. The oral cavity irradiation with a NCIR has been made during 100 or 300 s, in each 24 hours during 6 days after contamination. A concentration of C. albicans in the oral cavity was detected at the 7th day of contamination (in 24h after the last irradiation). An assessment of a free-radical homeostasis of animals after a NCIR treatment was made according to the blood plasma and erythrocyte free-radical reaction level, which was studied with a method of induced chemiluminescence.
Results. Both bacteriostatic and biocydic effects of the spark discharge gas discharge plasma NCIR, as well as an increase of the free-radical process level in experiments in vitro and in vivo are established. It is revealed, that a NCIR of the gas discharge plasma possesses of 100% of a bactericidal and fungicidal effect at the bacterial suspension treatment during 200 s in experiments in vitro. A bacteriostatic effect of a NCIR is demonstrated in experiments in vivo. The free-radical process level after a NCIR effect was increased in the cell suspension in experiments in vitro, that radiation in a microorganism activates the free-radical processes at the erythrocyte membranes in the studied modes.
