The Use of Cytomorphological Method to Assess Spermatogenesis in Hypoxia
The aim of the investigation is to study the assessment possibilities of spermatogenesis after acute hypobaric hypoxia using cytomorphological investigation of spermary tissue.
Materials and Methods. The experiments were carried out on 50 mature male non-pedigree white rats weighing 230–250 g. Acute hypoxia was simulated in flow low-pressure chamber.
Conclusion. Cytomorphological investigation of spermary tissue enables to state that after acute hypobaric hypoxia there occur marked changes of spermary cell structure, the number of all cell populations of seminiferous epithelium decreases, including Sertoli cells and Leydig cells. It is indicative of serious disorders of spermatogenesis.
- Ruzen-Range E. Spermatogenez u zhivotnykh [Spermatogenesis in animals]. Moscow: Mir; 1980; 255 p.
- Hess R.A., Franca R.L. Spermatogenesis and cycle of the seminiferous epithelium. In: C.Y. Cheng (edit.). Molecular mechanisms in spermatogenesis. Austin, TX: Landes Bioscience/Springer Science; 2008; p. 1–15.
- Clermont Y. Kinetics of spermatogenesis in mammals: seminiferous epithelium cycle and spermatogonial renewal. Physiol Rev 1972; 52: 198–235.
- Agadzhanyan N.A. Funktsii organizma v usloviyakh gipoksii i giperkapnii [Body functions in conditions of hypoxia and hypercapnia]. Moscow: Meditsina; 1986; 272 р.
- Luk’yanova L.D. Bull Eksp Biol Med 1997; 9: 244–253.
- Ivanov Yu.V. Gigiena i sanitariya 1986; 4: 52–55.
- Potemina T.E., Artifeksov S.B. Sposob kolichestvennoy otsenki effektivnosti spermatogeneza [The method of quantitative estimation of spermatogenesis effectiveness]. Patent RF 2231979. 2004.
- Metodicheskie rekomendatsii po eksperimental’nomu izucheniyu preparatov, predlagaemykh dlya klinicheskogo izucheniya v kachestve antigipoksicheskikh sredst [Methodological recommendations on experimental study of pharmaceuticals suggested for clinical studies as antihypoxic agents]. Pod red. L.D. Luk’yanovoy [L.D. Luk’yanova (editor)]. Moscow: Meditsina; 1990; 18 р.
- Reproduktivnaya endokrinologiya [Reproductive endocrinology]. Pod red. S.S.K. Yena, R.B. Dzhaffe [S.S.K. Yena, R.B. Dzhaffe (editor)]. Moscow: Meditsina; 1998; 704 p.
- Pshenichnikova T.Ya. Besplodie v brake [Infertility within marriage]. Moscow: Meditsina; 1991; 317 p.
- Cheng C.Y., Mruk D.D. Cell junction dynamics in the testis: Sertoli–germ cell interactions and male contraceptive development. Physiol Rev 2002; 82: 825–874.
- Cheng C.Y., Mruk D.D. The biology of spermatogenesis: the past, present and future. Phil Trans R Soc 2010; 365(1546): 1459–1463.