The Study of Biocidal Mechanisms of Spark Discharge Plasma Radiation
The aim of the investigation is to study the biocidal mechanisms of spark discharge plasma radiation.
Materials and Methods. The suspensions of studied bacterial strains were treated in optimal discharge conditions: pulse capacitor capacity C=3.3 nF, ballast resistance R=10 MOhm, power supply voltage UPS=11 kV, pulse recurrence frequency — 10 Hz.
Biocidal effect of plasma radiation was estimated by the number of colony-forming units. The analysis of oxidative process intensity in procariotic cells after plasma radiation exposure was performed according to relative concentration of lipid peroxidation products and fluorescence level of bityrosine, tryptophan and glycated proteins, cell membrane hydrophoby change was studied by fluorescence intensity of 1.6-diphenyl-1,3,5-hexatriene. The character of metabolic changes in cells after plasma radiation exposure was studied by pyridine nucleotides fluorescence intensity, surface structures condition — by the concentration of sialic acids in extracellular medium. Extracellular pH change was assessed pH-metrically, and intracellular рH was analyzed by means of fluorometry, using fluoroscein probe.
Results. 100% gram-positive and gram-negative bacteria growth inhibition was found after plasma radiation exposure within 60 s. Surface carbohydrate structures of gram-positive bacteria were revealed to be destroyed to a greater degree. There was observed the increase of membrane and intracellular pH hydrophoby after the treatment of bacterial cells suspension. The level decrease of molecular products of lipid peroxidation was found. The proteins of gram-negative bacteria were shown to be exposed to more pronounced oxidative modification than those of gram-positive ones. Pyridine nucleotides in oxidized condition were found to prevail in cells after plasma radiation exposure.
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