Morphofunctional State of Peripheral Blood Erythrocytes after Femtosecond Laser Treatment
The aim of the investigation was to assess femtosecond laser effect on morphofunctional state in peripheral blood erythrocytes exposed to radiation flux density variations.
Materials and Methods. We determined the level of malondialdehyde (MDA), the activity of superoxide dismutase (SOD), catalase and glutathione-transferase, and assessed topology and rigidity of erythrocyte membranes using scanning probe microscopy. In the experiment we used erbium fiber laser with pulse duration of 82·10-15 s, peak and average power of 6 kW and 1.26 mW, respectively, and wavelength of 1.55 µm; radiation flux density being within the range from 0.10 to 2.70 J/cm2.
Results. MDA level indicating lipid peroxidation intensity was found to change slightly varying from 430 to 509 µmol/l. The activity of antioxidant enzymes — SOD and catalase was revealed to depend on the energy density: its most pronounced activity increase was recorded for the radiation flux density of 0.10 J/cm2. GT activity for all selected radiation energy densities was similar to that in control group. Scanning probe microscopy findings indicate erythrocyte topology modification as well as undulatory increase of erythrocyte membrane rigidity at increased energy density of femtosecond laser radiation.
- Prindeze N.J., Moffatt L.T., Shupp J.W. Mechanisms of action for light therapy: a review of molecular interactions. Exp Biol Med (Maywood) 2012; 237(11): 1241–1248.
- Nechipurenko N.I., Pashkovskaya I.D., Stepanova Yu.I., Vasilevskaya L.A. Mekhanizmy deystviya i biologicheskie effekty nizkointensivnogo lazernogo izlucheniya [Mechanisms of action and biological effects of low-intensity laser radiation]. Meditsinskie novosti — Medical News 2008; 12: 17–21.
- Portillo Muñoz M., Lorenzo Luengo M.C., Sánchez Llorente J.M., Peix Sánchez M., Albaladejo A., García A., Moreno Pedraz P. Morphological alterations in dentine after mechanical treatment and ultrashort pulse laser irradiation. Lasers Med Sci 2012; 27(1): 53–58.
- Perin B., Zaric B., Jovanovic S., Matijasevic J., Stanic J., Kopitovic I., Zvezdin B., Antonic M. Patient-related independent clinical risk factors for early complications following Nd: YAG laser resection of lung cancer. Ann Thorac Med 2012; 7(4): 233–237.
- Rakityanskiy M.M., Agranat M.B., Amitkov S.I. Issledovanie biologicheskikh ob”ektov na kletochnom i subkletochnom urovne s pomoshch’yu femtosekundnogo lazernogo opticheskogo pintseta-skal’pelya [The study of biological objects at the cellular and subcellular level using femtosecond laser optical forceps-scalpel]. Vestnik transplantologii i isskustvennykh organov — Vestnik of Transplantology and Artificial Organs 2009; 3: 31–36.
- Girard B., Yu D., Armstrong M.R. Effects of femtosecond laser irradiation on osseous tissues. Lasers Surg Med 2007;3: 273–285.
- Thögersen J., Knudsen C.S., Maetzke A. Reproductive death of cancer cells induced by femtosecond laser pulses. Int J Radiat Biol 2007; 5: 289–299.
- Ezhov V.V., Torchinov A.M., Geynits A.V., Firichenko V.I. Nekotorye biofizicheskie aspekty kontaktnoy IK-lazernoy terapii sheyki matki [Some biophysical aspects of contact IR laser therapy of cervix uteri]. Lazernaya meditsina — Laser Medicine 2008; 12(3): 15–17.
- Ribeiro M.M., Franquelim H.G., Torcato I.M., Ramu V.G., Heras M., Bardaji E.R., Castanho M.A. Antimicrobial properties of analgesic kyotorphin peptides unraveled through atomic force microscopy. Biochem Biophys Res Commun 2012; 420(3): 676–679.
- Freitinger Skalická Z., Zölzer F., Beránek L., Racek J. Indicators of oxidative stress after ionizing and/or non-ionizing radiation: Superoxid dismutase and malondialdehyde. J Photochem Photobiol B 2012; 117: 111–114.
- Korsi L.V., Sokolov V.G. Lazernyy sposob fotokhimicheskoy destruktsii opukholey bez ekzogennykh sensibilizatorov. V kn.: Lazerno-opticheskie sistemy i tekhnologii [Laser method of tumor photochemical destruction without exogeneous sensibilizer. In: Laser optical systems and technologies]. Moscow: FGUP “NPO Astrofizika”; 2009; 101–106.
- Pleskova S.N., Zaslavzkaja M.I., Guschina Yu.Yu., Koksharov I.A., Erastova Yu.G. Morphological investigation of human blood neutrophil phagocytosis in vitro by AFM. Phys Low-Dim Struct 2001; 3: 229–236.
