Oxidative Stress Level and Anthropogenic Load Index as Prognostic Criteria of Disease Outcome in Patients with Oropharyngeal Cancer

The aim of the investigation was to assess the role of ecological problems and oxidative homeostasis parameters in life expectancy prognosis of oncology patients in the territories with different anthropogenic load level.

Materials and Methods. A group of oncology patients included 80 males with oropharyngeal tumors (III–IV stage tumors of oral and oropharyngeal cavity), mean age being 58.63±0.68. According to clinical and morphological characteristics of the disease, and social and adaptation criteria all the patients were referred to a homogeneous group. A control group consisted of 10 men from different territories with no oncology in past history.

Results. Pro-, antioxidant system components were found to be the markers of latent inhomogeneity in a group of patient with III–IV stage oropharyngeal tumors. Oxidative stress level in blood of patients with similar clinicopathologic and anatomical characteristics appeared to be different. Regression analysis findings showed the life expectancy in patients with III–IV stage oropharyngeal tumors to be associated with anthropogenic load index of residence place, red blood cell superoxide dismutase activity, maximum chemiluminescence intensity, TNF-α concentration, ketondinitrophenylhydrazine level in induced oxidation and malondialdehyde concentration in blood plasma.

Conclusion. The study has demonstrated for the first time the relationship of environmental factor and oxidative homeostasis parameters, and their integrated effect on life expectancy of oncology patients.

1. Zaridze D.G. Epidemiologiya, mekhanizmy kantserogeneza i profilaktika raka [Epidemiology, mechanisms of carcinogenesis, and cancer prevention]. Arh Patol — Pathology Archives 2002; 2: 53–61.
2. Protsenko S.A. Poiski putey individualizatsii protivoopukholevoy terapii. [Looking for ways of antitumor therapy individualization]. Prakticheskaya onkologiya — Practical Oncology 2007; 8(4): 173–181.
3. Zenkov N.K., Lankin V.Z., Men’shchikova E.B. Okislitel’nyy stress: biokhimicheskiy i patofiziologicheskiy aspekty [Oxidative stress: biochemical and pathophysiological aspects]. Moscow; 2001; 343 p.
4. Diehn M., Cho R.W., Lobo N.A., et al. Association of reactive oxygen species levels and radioresistance in cancer stem cells. Nature 2009; 458(7239): 780–783.
5. Karihtala P., Kauppila S., Puistola U., Jukkola-Vuorinen A. Divergent behaviour of oxidative stress markers 8-hydroxydeoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal (HNE) in breast carcinogenesis. Histopathology 2011; 58(6): 854–862.
6. Sau A., Pellizzari Tregno F., Valentino F., Federici G., Caccuri A.M. Glutathione transferases and development of new principles to overcome drug resistance. Archives of Biochemistry and Biophysics 2010; 500(2): 116–122.
7. Prin’kova T.Yu., Prokhorova V.I., Khot’ko E.A., Tsyrus’ T.P., Shishlo L.M., Taganovich A.D. Laboratornye pokazateli endogennoy intoksikatsii pri rake tela matki i znachenie ikh opredeleniya dlya otsenki stadii i stepeni differentsirovki opukholi [Laboratory findings of endogenous intoxication in hysterocarcinoma and the significance of their determination for the assessment of tumor stage and differentiation]. Laboratornaya diagnostika. Vostochnaya Evropa — Laboratory Diagnostics. Eastern Europe 2012; 4: 79–87.
8. Srivastava S., Natu S.M., Gupta A., Pal K.A., Singh U., Agarwal G.G., Uma Singh, Goel M.M., Srivastava A.N. Lipid peroxidation and antioxidants in different stages of cervical cancer: prognostic significance. Indian Journal of Cancer 2009; 46(4): 297–302.
9. Gidranovich A.V. Prognozirovanie raka molochnoy zhelezy na osnovanii biokhimicheskikh pokazateley syvorotki krovi [Breast cancer prognosis based on blood serum biochemical measurements]. Novosti khirurgii — Surgery news 2012; 20(4): 64–69.
10. Ambrosone C.B., Ahn J., Singh K.K., Rezaishiraz H., Furberg H., Sweeney C., Coles B., Trovato A. Polymorphisms in genes related to oxidative stress (MPO, MnSOD, CAT) and survival after treatment for breast cancer. Cancer Res 2005; 65(3):1105–1111.
11. Maslennikova A.V., Stcherbatyuk T.G., Lazareva V.A., Davydenko D.V. Prognosticheskoe znachenie parametrov prooksidantnogo–antioksidantnogo statusa bol’nykh mestno-rasprostranennym rakom polosti rta i glotki [Prognostic value of prooxidant-antioxidant status parameters of patients with locally advanced oropharyngeal cancer]. Med Al’m — Medical Almanac 2009; 3(8): 110–115.
12. Gelashvili D.B., Basurov V.A., Rozenberg G.S. Ekologicheskoe zonirovanie territoriy s uchetom roli sokhranivshikhsya estestvennykh ekosistem. [Ecological zoning of territories in terms of preserved natural ecosystems]. Povolzhskiy ekologicheskiy zhurnal — Povolzhsky ecological journal 2003; 2: 99–109.
13. Kuz’mina E.I., Nelyubin A.S., Shchennikova M.K. Primenenie indutsirovannoy khemilyuminestsentsii dlya otsenki svobodnoradikal’nykh reaktsiy v biologicheskikh substratakh. V kn.: Biokhimiya i biofizika mikroorganizmov [The use of induced chemiluminescence to assess free radical reactions in biotic substrates. In: Biochemistry and Biophysics of Microorganisms]. Gorky; 1983; p. 179–183.
14. Ermolin S.V. Biokhemilyuminometr. BKhL-07. V kn.: Aktivnye formy kisloroda, oksid azota, antioksidanty i zdorov’e cheloveka [Biochemiluminometer. БХЛ-07. In: Reactive oxygen species, nitric oxide, antioxidants and human health]. Smolensk; 2005; p. 21–23.
15. Levine R.L. Carbonyl modified proteins in cellular regulation, aging and disease. Free Radic Biol Med 2002; 32: 790–796.
16. Dubinina E.E. Okislitel’nye modifikatsii belkov syvorotki krovi cheloveka, metod ee opredeleniya [Protein oxidative modifications of human blood serum, and blood serum estimation method]. Voprosy meditsinskoy khimii — Medical Chemistry Issues 1995; 1: 24–26.
17. Korolyuk M.A., Ivanova L.I., Mayorova I.G., Tokarev V.E. Metod opredeleniya aktivnosti katalazy [Catalase activity estimation method]. Laboratornoe delo — Laboratory Science 1988; 1: 16–19.
18. Rebrova O.Yu. Statisticheskiy analiz meditsinskikh dannykh. Primenenie prikladnykh programm STATISTICA [Statistical analysis of medical data. Application software STATISTICA]. Moscow: MediaSfera; 2002; 312 p.
19. O sanitarno-epidemiologicheskoy obstanovke v Nizhegorodskoy oblasti v 2010 godu: gosudarstvennyy doklad [On sanitary and epidemiologic situation in Nizhny Novgorod region in 2010: a state report]. Nizhny Novgorod: Upravlenie federal’noy sluzhby po nadzoru v sfere zashchity prav potrebiteley i blagopoluchiya cheloveka po Nizhegorodskoy oblasti; 2011; 314 p.

Stcherbatyuk Т.G., Davydenko D.V., Novikova V.А. Oxidative Stress Level and Anthropogenic Load Index as Prognostic Criteria of Disease Outcome in Patients with Oropharyngeal Cancer. Sovremennye tehnologii v medicine 2013; 5(4): 25