Morphological Changes of the Brain Tissue in Rats with Experimental Model of Ischemic Stroke in the Dynamics of Treatment by Immunobiological Preparation Cryocell-Cryocord
Investigations of angiogenesis biological characteristics in the post-infarction zone may be of great importance not only for understanding the causes of stroke development, but also for more qualitative treatment planning. And although prognostic and therapeutic principles are only being formed, achievements in understanding the processes of angiogenesis have already been used in clinical practice, and their assessment, perhaps, will be applied in routine clinical activities.
The aim of the investigation was to evaluate the possibilities of using immunobiological preparation Cryocell-Cryocord in the treatment of ischemic stroke on the basis of the findings obtained in the course of studying morphological characteristics of brain tissue and the condition of the rats cerebral capillaries in experiment.
Materials and Methods. 120 white outbred rats were used in our investigation. They were divided into 4 groups, with 30 animals in each: intact rats without focal cerebral ischemia (FCI) and treatment; rats after FCI and with no treatment; rats after FCI with standard treatment; and rats after FCI with standard treatment complemented by Cryocell-Cryocord. A comparative morphometric analysis of the sensorimotor cortex structural components and the newly formed blood vessels in the necrotic area has been carried out during the period of cerebral infarction progression. The ultrastructure has been examined by an electron microscope.
Results. Inclusion of the immunobiological preparation Cryocell-Cryocord into the standard treatment scheme for ischemic stroke has been found to trigger angiogenesis in the infarcted area and to preserve the blood-brain barrier structure, which favored the recovery of the lost functions.
Conclusion. The immunobiological preparation Cryocell-Cryocord has been shown to possess pronounced angioprotective properties and to stimulate endogenous angiogenesis, which allows it to be included into the treatment scheme for ischemic stroke.
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