Pathomorphological Assessment Method of Myocardial Infarction Age
The aim of the investigation was to develop a simple and available for practical application method of pathomorphological assessment of myocardial infarction age with a wide range of registered terms.
Materials and Methods. Specimens of the experimental myocardial infarction zone (a model of diathermocoagulation of the rat periconical interventricular artery; n=50) in the period from 2 h to 30 days, and post-mortem specimens of 30 men died of transmural myocardial infarction and post-infarction cardiosclerosis in the period from 6 h to 30 days were immunohistochemically investigated using antibodies to the matrix metalloproteinase 9 (MMP9).
Results. It has been established that localization of MMP9 in the infarction zone enables determining with a sufficiently high precision the time that passed from infarction onset. In fatal outcomes developed during several hours after infarction, the neutrophil cytoplasm in the infarction zone had intensive color. On day 1–2 a bright color of the extracellular matrix was noted. In later terms (days 3–21) staining of the fibroblastic cell line in the border zone (with maximum on days 7–14) was observed. The dynamics of MMP9 content and its localization in the myocardium of the dead patients corresponds to the data obtained in the experimental investigations.
Conclusion. The proposed method allows pathologists to clearly differentiate myocardial infarction age using pathomorphological investigations.
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