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Biological Response to a Novel Hybrid Polyoligomer: <i>in vitro</i> and <i>in vivo</i> Models

Biological Response to a Novel Hybrid Polyoligomer: in vitro and in vivo Models

Bokov A.E., Bulkin A.A., Davydenko D.V., Orlinskaya N.Yu., Egorikhina M.N., Rubtsova Yu.P., Charykova I.N., Kovylin R.S., Yudin V.V., Chesnokov S.A., Morozov A.G., Mlyavykh S.G., Aleynik D.Ya.
Key words: oligoester di(meth)acrylate; polylactide; hybrid porous polyoligomer; bone defect model; tissue remodeling; cytotoxicity; biocompatibility.
2020, volume 12, issue 6, page 36.

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The aim of the study is to evaluate biocompatibility of a novel hybrid polyoligomer in in vitro and in vivo models.

Materials and Methods. Cytotoxicity of the material was investigated using the MTT assay with human dermal fibroblasts as test cultures. To study direct interaction of the hybrid polyoligomer with cells, the fibroblasts were cultured on the polymer samples for 96 h, the cultures were assessed every 24 h using fluorescence microscopy. To study the tissue reaction in the area of contact with the donor bed and the morphological features of the implanted sample restructuring, a case-control study was performed using a rabbit model. Samples of hybrid polyoligomer were implanted into the bone defect formed in the left iliac crest in 10 rabbits. In the control group, the prepared allograft samples were transplanted into similar defects in 10 animals. The rabbits were sacrificed 4 and 8 weeks after the operation. The standard morphological methods with hematoxylin and eosin staining and immunohistochemical Ki-67 proliferation marker evaluation were used to assess the state of tissues in the defect area.

Results. The results demonstrate that the hybrid polyoligomer is not cytotoxic (cytotoxicity score 0–1), cells adhere well to its surface, retain their viability and typical morphology throughout the entire observation period. No negative impact of material implantation on the health state and behavior of animals was detected. Morphological examination showed the absence of inflammatory changes, formation of thin-walled capillary vessels, and considerable proliferative activity of mesenchymal cells in the defect area, even though it was more intense than in the control group.

Conclusion. No inflammation signs were detected by 8th week of the experiment. It was defined that new bone was beginning to form. The results of analysis support the conclusion that the developed hybrid materials are prospective for further research as potential bone substitute.

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