Lipid Metabolism and Ultrastructure of Hepatocytes in Postreperfusive Period at the Ozonized Physiologic Solution Use in Experiment
Aim of work is investigation of the ozonized physiologic solution (OPhS) influence on a lipid metabolism and ultrastructure of hepatocytes at a model of ischemia/reperfusion in experiment.
It is demonstrated, that a use of OPhS in the early postreperfusive period, differing from oxygenized physiologic solution, favors a reduction of a glucose utilization aerobic way, normalization of the energetically reserved lipid content, an increase of a phosphatidylserine content, a ratio of unsaturated and saturated phospholipids at the background of a cholesterol decrease and an increase of the membrane lipid bilayer fluidity, respectively, which improves the lipid-dependant membrane enzyme functioning conditions. A membrane-simulating effect of OPhS is manifested in decrease of the phosphatidylcholine and phosphatidyletanolamine lysoforms, the increased concentrations of which manifest a detergent-like property. The hepatocytes have a more conserved structure. Infusion of OPhS causes intensification of the lipid peroxidation processes and an increase of the antioxidant protection enzyme activity. However an increase of a lactate/pyruvate ratio relating to a final value testifies to the hypoxic focus presence and, consequently, to a possible development of the liver function disturbances in remote postischemic period and, correspondingly, to necessity of additional correction during a reduction period.
