Morphological Characteristic of the Mouth Cavity Mucous Membrane Regeneration Processes in Experiment at a Radiowave Method of Tissue Dissection Use
Aim of investigation is establishment of regularities (peculiarities) of the wound at a mouth cavity mucous membrane healing depending on a character of physical effect on the studied tissues in experiment.
Materials and Methods. Experimental investigation is made on 80 rat-males of Wistar line of the same age, with a mass of 350—420 g. A dissection of a buccal area mucous membrane portion, with a 0.5x0.7 cm size, was made in experimental animals under a local anesthesia (0.4 ml of a novocain solution 2%). Two groups of animals participated in experiment: in the 1st group a wound was applied with a common scalpel; in the 2nd group — with a radiowave device (″Surgitron Dentosurg″, Ellman firm), mode №2 (dissection and coagulation); a power — 4—4.5 units. The both group animal withdrawal from experiment was made under an ether effect, with investigating medium collection in 1, 3, 7, 24, 48 and 72h after wound simulation. The tissue sections were stained with hematoxylin-eosin according to Van-Hison. A period of observation was 3 days, which is stipulated by a low traumatism of the simulated wounds and a rapid healing (up to 3—4 days). A system of computer quantitative morphometry was used for analysis of cytological and histological preparations.
Results. A synthesis of fibroblasts, a granular tissue forming and maturation are observed at the earlier dates, at a radiosurgical method in rat tissues, and an area of tissue necrosis, i.e. a damaging effect at investigation, is practically absent. A delayed regeneration with the tissue edema phenomenon conservation and the circulation disorders in postoperative period are observed at a traditional use of the cutting tools.
Conclusion. The received results testify to expediency of a radiowave surgery method use at the surgical interventions in a mouth cavity mucous membrane.