New Method of the Coccus Quantitative Consideration in the Supracellular Formations — Clusters and Biofilm
Aim of investigation is elaboration of a new method of the coccal microorganism calculation in the clusters and biofilm and methods of that method use for the staphylococcal biofilm quantitative assessments.
Materials and Methods. The different strains of the golden and epidermal staphylococci: Staphylococcus aureus (strains 5983/2, 5663/2, 18A) and Staphylococcus epidermidis (strains 178M, 0309, 328/5) were used.
Results. The possibilities of a newly elaborated method of the microbe automated calculation in the biofilm or microbic clusters are demonstrated. A method permits: 1) to consider an absolute quantity of microbic cells in a digital picture of a microscopic preparation; 2) to consider a quantity of clusters with a detection of the microbic cell quantity; 3) to depict a distribution of the ″microbe/cluster″ ratios in a form of graph.
