Issue 4 (2010)

Aim of investigation is assessment of the ozonized physiologic salt solution low therapeutic concentration influence on a tumor medical pathomorphosis in experimental conditions.

Materials and Methods. An investigation is made on laboratory animals — white nonlinear female-rats (75 specimen) with a mass of 250±10 g. An experimental model of neoplasia was created by twisting of the RMC-1 mammary gland cancer strain subcutaneously into the right axillary cavity area. The animals were included into experiment on the 45 days after a tumor twisting, a volume of which at that moment was 6—8 cm³. A decapitation of animals and taking of a tumor tissue were made on the 11 days after the experiment beginning under an ether anesthesia. The ozonized physiologic salt solution was received by barbotage of a NaCl solution 0.9% with an ozonooxygenous mixture.

A histological investigation of a tumoral tissue was accomplished by a generally accepted method. An analysis of induced biochemiluminescence and a measurement of the tumor tissue homogenate lipoperoxidation product levels were included into biochemical investigations.

Results. A combined influence of the ozonized physiologic salt solution low therapeutic concentrations and a doxorubicin more expressively and destructively effected a tumoral tissue. A use of ozonized physiologic salt solution potentiated a doxorubicin antitumoral activity, which was manifested in a statistically more expressed depression of the tumoral cell mitotic activity and a decrease of their viable elements, causing the more profound manifestations of a therapeutic pathomorphosis.