The Study of the Activity of Lipid Peroxidation, Antioxidant System and Ultrastructure of Organs in a Long-term Postresuscitative Period

The aim of the investigation is to study experimentally the change of the lipid peroxidation processes, the activity of superoxide dismutase, catalase, the content of the substrates of the carbohydrate metabolism, and the brain, heart and liver ultrastructure in rats on the 30th day of the postresuscitative period.

Materials and Methods. The experiments were carried on white nonlinear male rats. Clinical death was simulated by clamping cardiovascular bundle. The stagnation of the circulation was 10 min. The organs of the anesthetized animals were taken on the 40th minute (n=12) and on the 30th day of the postresuscitative period (n=8).

Results. Lipid peroxidation products (conjugated trienes in the brain and the liver and malonic dialdehyde in the myocardium) were shown to increase on the 30th day of the postperfusion period compared to the control group (40 min) against the background of the activity decrease of superoxide dismutase in all the studied organs. The lactate/pyruvate ratio exceeded the baseline. The mitochondria with an indistinct or damaged outer membrane, and the disorientation of the cristas were demonstrated by an electron-microscopic research. The findings indicated membrane destruction processes on the 30th postresuscitative day. The mentioned changes increase the risk of irreversible damage of membrane structures, and therefore, multi-organ dysfunction in a long-term postresuscitative period.

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Andreeva N.N., Soloviyova T.I., Balandina M.V., Yakovleva E.I. The Study of the Activity of Lipid Peroxidation, Antioxidant System and Ultrastructure of Organs in a Long-term Postresuscitative Period. Sovremennye tehnologii v medicine 2012; (2): 37