The Study of Intermolecular Interactions Using Optical Biosensors Operating on the Effect of Surface Plasmon Resonance

Present knowledge of the technology of optical biosensors based on the effect of surface plasmon resonance (SPR) is presented. SPR enables to register almost all molecular interactions in real time without any labels or associated processes. There are considered the main technical characteristics of SPR biosensors and the principles of analysis of kinetic, equilibrium and thermodynamic parameters of intermolecular interactions.

There are presented a number of examples of Biacore biosensors (GE Healthcare, USA) application in post-genomic researches carried out in the Institute of Biomedical Chemistry (Moscow, Russia): SPR technology has been used in the analysis of intermolecular interactions, like protein-protein, protein-low-molecular-weight compounds, protein-DNA; in the analysis of protein oligomerization and the search of dimerization inhibitors; in the search of drug prototype compounds interacting with a target protein; and in biosensor analysis of disease biomarkers.

The technology of optical biosensors operating on the effect of surface рlasmon resonance is getting one of the approaches in researching various intermolecular interactions. It is of universal character and enables to record the interactions between almost any molecular objects in a wide range of concentrations. SPR-biosensors have very high accuracy, capability of reproducing and sensitivity; due to these characteristics their application is growing both in fundamental and applied researches.

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Ivanov А.S. The Study of Intermolecular Interactions Using Optical Biosensors Operating on the Effect of Surface Plasmon Resonance. Sovremennye tehnologii v medicine 2012; (4): 142