Morphofunctional Status of Erythrocytes in Cervical Cancer Patients after Femtosecond Laser Radiation Exposure
The aim of investigation was morphofunctional assessment of peripheral erythrocytes in cervical cancer patients (Ib–IIa stage) after femtosecond laser radiation exposure with different energy density.
Materials and Methods. We assessed membrane cytoarchitectonics, rigidity and the parameters of “lipid peroxidation–antioxidants” system using scanning probe microscopy and biochemistry. Femtosecond laser radiation with wavelength of 1.55 µm at dose from 0.10 to 2.7 J/cm2 was used.
Results. Femtosecond laser radiation in donors’ erythrocytes at specified doses was found to have no effect on the parameters of “lipid peroxidation–antioxidants” system, but dose-dependently it causes their reversible (echinocytes) and irreversible (spherocytes) transformation on the background of membrane rigidity increase. Malondialdehyde level in unchanged activity of antioxidant system enzymes increases significantly in erythrocytes of cervical cancer at Ib–IIa stage under exposure dose of 0.96–2.7 J/cm2 that can indicate an oxidative stress formation. In this respect, these doses of femtosecond laser radiation are not recommended for Ib–IIa stage cervical cancer patients.
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