High Frequency Radio Waves — an Innovative Approach to the Correction of Age-Related Skin Changes: Clinical and Immunohistochemical Research
The aim of investigation was to evaluate the efficiency of high frequency radio wave exposure (4.0 MHz) for the correction of age-related changes of facial skin by studying aspects of its regeneration, on the basis of morphological and immunohistochemical characteristics.
Materials and Methods. The study consisted of two parts. The clinical part included an analysis of the results of high frequency radio wave facial rejuvenation (4.0 MHz) (5 procedures: on days 1, 30, 60, and months 6, 12). Immunohistochemical investigation was performed on repeated punch-biopsy specimens of the skin behind the ear, before the exposure, and at 30, 60, 180 and 360 days after a single exposure. Collagen of types 1 and 3, elastin, Ki-67, CD34, SMA, MMP2 and TIMP1 were identified.
Results. It was found that the most evident morphological changes after high frequency radio wave exposure occurred in the deeper dermis layers and in the adjacent adipose tissue. Such remodeling of the extracellular matrix of the dermis causes an expansion of the deep dermis layers with the accumulation of collagen of types 1 and 3 with a greater proportional increase in favor of type 1. The key anti-ageing factor of radio wave exposure is considered to be the activation of neoangiogenesis in the dermis, which occurs gradually, reaching its maximum by month 12 after a single exposure.
- Belenky I., Elman M., Bar-Yosef U., Paun S.D., Vashkevich M., Levit K. Radio frequency therapy in esthetic medicine (review). Plasticheskaya khirurgiya i kosmetologiya 2011(4): 705–717.
- Lipova E.V., Glazko I.I. Physiological fundamentals of the application of “RF” and radio wave technologies in cosmetology. Apparatnaya kosmetologiya i fizioterapiya 2012; 3: 26–40.
- Stupin V.A., Smirnova G.O., Manturova N.E., Khomyakova E.N., Kogan E.A., Polivoda M.D., Silina E.V., Sinelnikova T.G. Comparative analysis of healing processes of surgical wounds in use of various kinds of radio-frequency cutting devices and a metal scalpel. Kurskiy nauchno-prakticheskiy vestnik “Chelovek i ego zdorov’e” 2010; 4: 9–14.
- Petrou I. RF device’s steady vs. pulsed waves heat the way to less painful facial rejuvenation. Cosmetic Surgery Times 2009; 12: 28–29.
- Rusciani A., Curinga G., Menichini G., Alfano C., Rusciani L. Nonsurgical tightening of skin laxity: a new radiofrequency approach. J Drugs Dermatol 2007; 6(4): 381–386.
- Trufanov V.D., Ivanov S.Yu., Kogan E.A., Fajzullina N.M Radiofrequency scalpels in maxillofacial and plastic surgery: experimental, immunohistochemical research. Institut stomatologii 2015; 2: 90–93.
- Maness W.L., Roeber F.W., Clark R.E., Cataldo E., Riis D., Haddad A.W. Histologic evaluation of electrosurgery with varying frequency and waveform. J Prosthet Dent 1978; 40(3): 304–308, http://dx.doi.org/10.1016/0022-3913(78)90037-9.
- Yutskovskaya Ya.A., Tarasenkova M.S., Kizey I.N., Trufanov V.D. Radio frequency lifting in a programme of anti-ageing therapy. Esteticheskaya meditsina 2010; 9(2): 3–10.
- Yutskovskaya Ya.A., Kizey I.N., Tarasenkova M.S., Trufanov V.D. Combination of radio wave skin lifting (Pelleve) and BT-A Injections in complex programmesfor correcting involution skin changes. Esteticheskaya meditsina 2010; 9(4): 453–457.
- Yutskovskaya Y., Kogan E., Leshunov E. A randomized, split-face, histomorphologic study comparing a volumetric calcium hydroxylapatite and a hyaluronic acid-based dermal filler. J Drugs Dermatol 2014; 13(9): 1047–1052.
- Education guide. Immunohistochemical staining methods, fourth edition. Key M. (editor). Carpinteria, California: Dako 2006.
- Ellinidi V.N., Anikeeva N.V. Prakticheskaya immunogistotsitokhimiya [Practical immunohistochemistry]. Saint Petersburg: VTsERM MChS Rossii; 2002.
