The Relation of Biological Properties of the Silk Fibroin/Gelatin Scaffolds with the Composition and Fabrication Technology

The aim of the investigation was to research the effect of preparation method and composition of silk fibroin and gelatin scaffolds on biological properties.

Materials and Methods. Silk fibroin, gelatin and their blend with different mass ratio scaffolds were prepared by electrospinning. To research scaffold’s structure light microscopy, scanning electron microscopy and confocal laser scanning microscopy were applied. Adhesion and proliferation of mice fibroblast 3T3 cell line were investigated to test biocompatibility of constructed scaffolds.

Results. Optimal parameters of device and fiber obtaining parameters were selected. Fibrous porous three-dimensional structure of investigated scaffolds was revealed. It was established that cell proliferative activity on electrospun scaffolds was significantly higher than on casting films. Addition of gelatin to scaffold composition increases cell proliferation.

Conclusions. Electrospun silk fibroin/gelatin scaffolds contain such polymers with mass ratio equal to 1:3 have significant greater ability to maintain cell and proliferation than fibroin and gelatin scaffolds.

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Sokolova A.I., Bobrova M.M., Safonova L.A., Agapova O.I., Moisenovich M.M., Agapov I.I. The Relation of Biological Properties of the Silk Fibroin/Gelatin Scaffolds with the Composition and Fabrication Technology. Sovremennye tehnologii v medicine 2016; 8(3): 6, https://doi.org/10.17691/stm2016.8.3.01