Migration of Human Dendritic Cells in vitro Induced by Vaccines Stimulating Humoral and Cell Immunity

Dendritic cells (DC) are specialized antigen-presenting cells. One of their function is to deliver antigens from peripheral tissues to lymphoid organs by migration controlled by chemokines.

The aim of the investigation was to study the effect of vaccines stimulating cellular or humoral response on the expression of chemokine receptors CCR7, CXCR4 and CXCR5 on DC, and assess the motility of the cells and migration response on chemokines.

Materials and Methods. Immature DC derived from monocytes in vitro were incubated with vaccines or inflammatory mediators, and then assessed their maturation and studied CCR7 gene expression, the presence of CCR7, CXCR4 and CXCR5 receptors on the outer membrane, spontaneous cell motility and chemotaxis induced by chemokines CCL21 and CXCL13.

Results. BCG tuberculosis vaccine stimulating cellular immune response effectively induces DC maturation, and has no effect on the expression of receptors CXCR4 and CXCR5 causing slight but reliable enhanced expression of gene and receptor CCR7 as well as chemotaxis induced by chemokine CCL21. Recombinant yeast hepatitis B vaccine inducing humoral immune response causes partial DC maturation increasing significantly the expression of receptors CCR7, CXCR4 and CXCR5, but does not increase spontaneous cell motility and enhances weakly chemotaxis in response to CCL21 and CXCL13.

Conclusion. Tuberculosis vaccine and hepatitis B vaccine induce different sets of chemokine receptors on DC, however, they stimulate DC hemotaxis relatively weakly. The findings suggest feasibility of searching new adjuvants, which enable to enhance the migration of DC carrying antigens to lymphoid organs.

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Talayev V.Y., Talaeva М.V., Voronina Е.V., Babaykina О.N. Migration of Human Dendritic Cells in vitro Induced by Vaccines Stimulating Humoral and Cell Immunity. Sovremennye tehnologii v medicine 2016; 8(3): 91, https://doi.org/10.17691/stm2016.8.3.10