The Study of Biochemical Parameters of Liver Mitochondria as Markers of Hypoxia in Burn Regeneration after Experimental Thermal Injury

The aim of this work was to study pro- and antioxidant systems and energy-generating functions of mitochondria in case of combined thermal injury.

Materials and Methods. The experiment was carried out on male rats of Wistar line. Two groups were formed: control group 1 (n=10) of intact healthy animals; experimental group 2 (n=10) of animals with combined thermal injury (20% contact burns and thermal inhalation impact of hot air and combustion products). Animals were taken out of the experiment on days 1, 7, and 14 post-injury by decapitation under anesthesia (Zoletil 100 + XylaVET).

Mitochondria were obtained by differential centrifugation. For the mitochondria identification, an electron microscopic study was conducted. In the liver mitochondria, the intensity of free radical oxidation, the activity of catalase, superoxide dismutase, succinate dehydrogenase, and cytochrome c oxidase were evaluated. The research results were processed using Statistica 6.0 (StatSoft Inc., USA).

Results. An increase of intensity of free radical oxidation in the liver mitochondria on days 7 and 14 post-injury was registered. Meanwhile, total antioxidant activity of blood plasma and catalase activity in erythrocytes in case of thermal injury were decreasing on all test days after the burn as compared to the control group.

Towards days 7 and 14, superoxide dismutase activity significantly decreased in comparison with the healthy animals. The study of succinate dehydrogenase and cytochrome c oxidase showed a decrease in specific activity of enzymes in the liver mitochondria on days 1, 7, and 14 after combined thermal injury. The most pronounced decrease in the activity of succinate dehydrogenase and cytochrome c oxidase was observed on day 14 after the burn.

Conclusion. A presence of oxidative stress during combined thermal injury was revealed, as well as a comprehensive mechanism of its formation, implicating both activation of free radical oxidation and decrease in antioxidative capacity imbalance in the functioning of prooxidant and antioxidant systems of the body. Inhibition of energy supply of cells, reduction in the cell aerobic and increased anaerobic oxidation were revealed.

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Soloveva A.G., Kulakova K.V., Orlinskaya N.Yu., Galova E.A. The Study of Biochemical Parameters of Liver Mitochondria as Markers of Hypoxia in Burn Regeneration after Experimental Thermal Injury. Sovremennye tehnologii v medicine 2018; 10(1): 81, https://doi.org/10.17691/stm2018.10.1.10