Features of Primary Hippocampal Cultures Formation on Scaffolds Based on Hyaluronic Acid Glycidyl Methacrylate

Mishchenko T.A., Mitroshina Е.V., Kuznetsova A.I., Shirokova O.M., Khaydukov E.V., Savelyev A.G., Popov V.K., Zvyagin A.V., Vedunova M.V.

Key words: scaffold; hyaluronic acid; biocompatibility; calcium imaging; primary hippocampal cultures.

2018, volume 10, issue 1, page 103.

DOI: https://doi.org/10.17691/stm2018.10.1.13

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The aim of the study was to investigate the morphological and metabolic features of primary hippocampal cultures formation on hydrogel films and scaffolds based on hyaluronic acid glycidyl methacrylate.

Materials and Methods. Hydrogel films and scaffolds with certain architectonics were developed by micromolding technique on the basis of hyaluronic acid glycidyl methacrylate. Primary hippocampal cells obtained from С57ВL/6 mouse embryos (E18) were cultured on the created constructs more than 14 days. Testing cell viability, morphometric assessment, and analysis of spontaneous calcium activity of primary hippocampal cultures were performed on day 14 of cultures development in vitro.

Results. This study revealed that the material for the development of scaffolds with given architectonics is non-toxic for the nervous system cells. Dissociated hippocampal cells were actively attached to the scaffold surface and were assembled into cell conglomerates, which exhibited spontaneous calcium activity.

Conclusion. Scaffolds designed on the basis hyaluronic acid glycidyl methacrylate have a high biocompatibility with the nervous system cells. Architectonics and adhesive properties of scaffold contribute to the formation of functionally active cell conglomerates.

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Mishchenko T.A., Mitroshina Е.V., Kuznetsova A.I., Shirokova O.M., Khaydukov E.V., Savelyev A.G., Popov V.K., Zvyagin A.V., Vedunova M.V. Features of Primary Hippocampal Cultures Formation on Scaffolds Based on Hyaluronic Acid Glycidyl Methacrylate. Sovremennye tehnologii v medicine 2018; 10(1): 103, https://doi.org/10.17691/stm2018.10.1.13