Field Potentials Recording in the Brain Tissue as a New Criterion for Determination of Glial Tumors Boundaries

The aim of the study was to evaluate the electrophysiological activities of tumor and peritumoral tissues in an experimental glioblastoma model and rationalize their use for determining the tumor boundaries.

Materials and Methods. Following methods were used in this study: extracellular electrophysiological activity (field postsynaptic potentials) registration; astrocytic glutamate currents patch-clamp registration; magneto-resonant imaging; surgical techniques in vivo. The experiments were performed using cell cultures (primary murine astrocyte culture and constant cell line cultures of rat glioma cells 2211), acute murine hippocampal slices, and rats with transplanted malignant glioma 101.8.

Results. A number of electrophysiological parameters of neurons and glial cells were evaluated using cell cultures, acute hippocampal slices, and the rat brain as a whole. Field postsynaptic potentials in the brain tissue differed between the non-injured areas and the zone of glioma development. No field potentials detected in the tumoral area, indicating the lack of excitability in the tumor cells.

Conclusion. Field potentials recorded in the brain tissue in vivo can be used as a novel criterion for determining the glial brain tumors boundaries.

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Mishchenko M.A., Lebedeva A.V., Mishchenko Т.А., Yashin K.S., Lepekhina L.S., Astafyeva K.A., Ivanova I.P., Vedunova М.V., Medyanik I.A., Kazantsev V.B. Field Potentials Recording in the Brain Tissue as a New Criterion for Determination of Glial Tumors Boundaries. Sovremennye tehnologii v medicine 2018; 10(3): 32, https://doi.org/10.17691/stm2018.10.3.4