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Современные технологии фиксации биологического материала, применяемые при проведении иммуногистохимических исследований (обзор)

Современные технологии фиксации биологического материала, применяемые при проведении иммуногистохимических исследований (обзор)

И.П. Григорьев, Д.Э. Коржевский
Ключевые слова: фиксация биологического материала; иммуногистохимия; формалин; этанол; глутаральдегид; соли цинка; цинк-этанол-формальдегид; тепловое демаскирование антигенов.
2018, том 10, номер 2, стр. 156.
DOI: https://doi.org/10.17691/stm2018.10.2.19

Полный текст статьи

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На основе собственного опыта и данных литературы рассмотрены современные способы фиксации биологического материала, используемые при проведении иммуногистохимических исследований. Среди необходимых свойств иммуногистохимического фиксатора на первом месте должны быть способность обеспечивать сохранность структуры ткани при наименьшем влиянии на антигенные свойства макромолекул. С этой точки зрения в обзоре анализируется применимость для иммуногистохимии ряда фиксаторов, широко используемых в гистологической, цитологической и иммуногистохимической практике: альдегидов (формальдегид, глутаральдегид, глиоксаль), обезвоживающих (коагулирующих) фиксаторов (этанол, метанол, ацетон), комбинированных фиксирующих растворов (жидкость Буэна, жидкость Карнуа, метакарн и др.), а также современных цинксодержащих фиксаторов и коммерческих продуктов, предлагаемых для фиксации биологических образцов. Использование большинства фиксаторов ведет к нарушению третичной и четвертичной структуры многих белков, что требует для их выявления с помощью иммуногистохимии дополнительной процедуры демаскирования эпитопов с помощью протеолитических ферментов или повышенной температуры. На основании анализа данных иммуногистохимических исследований различных антигенов отмечено высокое качество нового цинксодержащего фиксатора — цинк-этанол-формальдегида. Тем не менее сделан вывод, что ни один из известных на сегодняшний день фиксаторов не обладает таким сочетанием свойств, которые бы позволяли получать гистологические препараты высокого качества и при этом обеспечивали возможность выявления любых антигенов в изучаемой ткани.

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