A New Estimation Method of Blood Metabolism Dynamics of Patients with Heat Injuries
The aim of the investigation is to develop a new way of the estimation of blood metabolism dynamic of patients with heat injuries that allows detecting early disturbances of energy reactions.
Materials and Methods. There has been examined the blood of 20 patients aged from 20 to 60 years with burn area of 25–50% of body surface (II–III A, B degrees of injury). The activity of lactate dehydrogenase in erythrocytes hemolysate and the value of enzyme relation in direct and reverse reactions have been determined.
Results. There has been suggested a new estimation method of blood metabolism dynamics in patients with heat injuries. The technique determines lactate dehydrogenase activity and calculates a balance coefficient of energy reactions.
- Plakunov V.K. Osnovy enzimologii [Principles of Enzymology]. Moscow: Logos; 2001; 128 p.
- Zimin Yu.V., Syatkin S.P., Berezov T.T. Voprosy meditsinskoy khimii — Medical Chemistry Issues 2001; 3: 3–7.
- Messonnier L., Freund H., Feasson L. et al. Blood lactate exchange and removal abilities after relative high-intensity exercise: effects of training in normoxia and hypoxia. Eur J Appl Physiol 2001; 84(5): 403–412.
- Halliwell C.M., Morgan G., Ou C.P., Cass A.E. Introduction of a(poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules. Anal Biochem 2001; 295(2): 257–261.
- Ivanova V.A., Petrash V.V., Tsimbalistov A.V., Sinitskiy A.A., Lopushanskaya T.A., Voytyatskaya I.V., Eysmont Yu.A. Sposob ekspress-otsenki dinamiki metabolizma sistemy krovi [Rapid evaluation technique of hemic system metabolism dynamics]. Patent RF No.2315306.
- Kochetov G.A. Prakticheskoe rukovodstvo po enzimologii [Enzymology Practical Guide]. Moscow: Vysshaya shkola; 1980; 272 p.
- Glants S. Mediko-biologicheskaya statistika [Biomedical Statistics]. Translated from English. Moscow: Praktika; 1999; 459 p.
- Solov’eva A.G., Zimin Yu.V. Sposob otsenki dinamiki metabolizma krovi u bol’nykh s termicheskoy travmoy [Estimation method of blood metabolism dynamics in patients with heat injuries]. Patent RF №2392865.