Molecular Epidemiological Monitoring of Streptococcus Pneumoniae Strains Isolated in Elderly Patients with Common-Acquired Pneumoniaes
The problem of microbiological monitoring and epidemiological surveillance over S. pneumoniae strains as one of ubiquitous agents causing common-acquired pneumoniaes and other respiratory infections of different severity is still urgent.
Multilocus sequence typing is a promising technique of molecular epidemiological monitoring enabling to identify epidemically dangerous clones of ubiquitous agents.
The aim of the investigation was to assess the spread of epidemically significant Streptococcus pneumoniae strainsby multi-locus sequence typing in elderly patients with common-acquired pneumoniae, bronchites, and agents, and identify a dominant genotype.
Materials and Methods. We studied 14 strains isolated in patients with common-acquired pneumoniaes (among them 7 — multi-resistant), 8 strains — in patients with chronic obstructive pulmonary disease, 4 strains — in the carriers. Multilocus sequence typing was carried out in accordance with M.C. Enright and B.G. Spratt technique (1998).
Results. All strains isolated in three populations are relative isolates of Streptococcus pneumoniae, and the majority of them (18 of 26) have a unique genotype determining the presence of one sequence type for each strain. From 14 strains isolated in elderly patients with common-acquired pneumoniae, 6 belonged to Taiwan 19F-14. Among the strains isolated in the carriers, the strain identical to R6 strain prevailed. No genotype was found to prevail among the strains isolated in patients with chronic obstructive pulmonary disease.
Conclusion. Multi-locus sequence typing enables to identify new genotypes of S. pneumoniaeand prognosticate the appearance of epidemically dangerous strains with new properties.
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