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Novel Biocompatible Material Based on Solid-State Modified Chitosan for Laser Stereolithography

Novel Biocompatible Material Based on Solid-State Modified Chitosan for Laser Stereolithography

Timashev P.S., Bardakova K.N., Demina Т.S., Pudovkina G.I., Novikov М.М., Markov М.А., Asyutin D.S., Pimenova L.F., Svidchenko Е.А., Ermakov А.М., Selezneva I.I., Popov V.К., Konovalov N.А., Akopova Т.А., Solovieva А.B., Panchenko V.Ya., Bagratashvili V.N.
Keywords: solid-state synthesis; laser stereolithography; chitosan; three-dimensional matrices; biocompatible materials; cytotoxicity; human mesenchymal stromal cells.
СТМ, 2015, volume 7, issue 3, pages 20-31.

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The aim of the investigation was to develop a novel biodegradable material based on chitosan synthesized by solid-state technoogy, and to create based on biocompatible three-dimensional cell-carrying scaffolds using laser stereolithography.

Materials and Methods. Reactive systems were developed based on chitosan grafted with allyl, polyethylene glycol diacrylate, and the photoinitiator Irgacure 2959. The structures were obtained using laser stereolithography setting LS-120 (Institute on Laser and Information Technologies, Russian Academy of Sciences, Russia).

Results. Partial replacement of chitosan amino groups by allyl groups (CТ-А) and the introduction of polyethylene glycol diacrylate (PEG-DA) as a crosslinking agent were found not to reduce the material biocompatibility. The metabolic activity determination of NCTC L929 cells using MTT assay showed that the samples under study to contain none water-soluble components toxic to mammalian cells. The samples based on CT-A and CT-A with a crosslinking agent PEG-DA are biocompatible and are able to support adhesion, spreading and proliferative activity of human mesenchymal stromal cells, but have significant differences in the extent and nature of the expression activation of gene markers for osteogenic differentiation path.

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Timashev P.S., Bardakova K.N., Demina Т.S., Pudovkina G.I., Novikov М.М., Markov М.А., Asyutin D.S., Pimenova L.F., Svidchenko Е.А., Ermakov А.М., Selezneva I.I., Popov V.К., Konovalov N.А., Akopova Т.А., Solovieva А.B., Panchenko V.Ya., Bagratashvili V.N. Novel Biocompatible Material Based on Solid-State Modified Chitosan for Laser Stereolithography. Sovremennye tehnologii v medicine 2015; 7(3): 20–31, http://dx.doi.org/10.17691/stm2015.7.3.03


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