The Content of Soluble HLA Class I and HLA-DR Molecules in Serum in Patients with Uterine Cervix and Body Pathology

The aim of the investigation was to assess the correlation of the content of soluble HLA (sHLAI) class I and HLA-DR (sHLA-DR) molecules in blood serum of patients with uterine cervix and body pathologies, and their pathology type, tumor grade, the number and localization of myomatous nodes.

Materials and Methods. 142 women with uterine cervix and body pathology aged 31–79 years (median — 52 years) were under study. Serum level of sHLAI and sHLA-DR molecules was determined by enzyme immunoassay using monoclonal antibodies. Blood samples were drawn from cubital vein. All tests were performed before and after the treatment course. For enzyme immunoassay we used mouse monoclonal antibodies to reveal soluble differentiated molecules.

Results. Malignant pathologies of uterine cervix and body and myomas were found to be accompanied by an increased serum level of sHLAI и sHLA-DR molecules, its degree depending on the type of pathology diagnosed in patients with cervical cancer and hysterocarcinomas, and differentiation degree of adenocarcinoma in hysterocarcinoma patients, the number and localization of myomatous nodes. Initial concentration of soluble HLA class I and HLA-DR molecules in a preoperative period can serve as an additional diagnostic test in patients with uterine tumors for their further selection for surgery.

1. Khaitov R.M., Alekseev L.P. Physiological role of human major histocompatibility complex. Immunologiya 2001; 3: 4–12.
2. Countini P., Zocchi M.R., Pierri I., Albarello A., Poggi A. In vivo apoptosis of CD8(+) lymphocytes in acute myeloid leukemia patients: involvement of soluble HLAI and Fas ligand. Leukaemia 2007; 21(2): 253–260, http://dx.doi.org/10.1038/sj.leu.2404494.
3. Nocito M., Montalban C., Gonzales-Porque P., Villar L.M. Increased soluble serum HLA class I antigens in patients with lymphoma. Hum Immunol 1997; 58(2): 106–111, http://dx.doi.org/10.1016/S0198-8859(97)00227-9.
4. Khudiakova N.E., Novikov V.V., Kravchenko G.A., Ivanova N.I., Ptitsyna I.S., Nosov N.N. Soluble HLA class I and HLA class II antigens in the blood of HIV infected patients. Zhurnal mikrobiologii, epidemiologii i immunobiologii 2004; 1: 42–45.
5. Kravchenko G.A., Novikov D.V., Ptitsyna Yu.S., Novikov V.V. Soluble immune cell membrane antigen levels in serum of carriers of viral hepatitis G markers. Voprosy virusologii 2005; 50(5): 19–22.
6. Novikov V.V., Evsegneeva I.V., Karaulov A.V., Baryshnikov A.Yu. Soluble immune cell membrane antigens in socially important infections. Rossiyskiy bioterapevticheskiy zhurnal 2005; 4(2): 100–105.
7. Lebedev M.Ju., Vilkov S.A., Sholkina M.N., Krizhanova M.A., Novikov V.V., Vyasmina E.S., Baryshnikov A.Ju. Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients. Burns 2003; 29(2): 123–128, http://dx.doi.org/10.1016/S0305-4179(02)00245-0.
8. Novikov V.V., Karaulov A.V., Baryshnikov A.Yu., Kravchenko G.A., Babaev A.A., Gostyuzhova E.A., Evsegneeva I.V. The characteristics of structural condition of soluble immune cell membrane antigen pool. Molekulyarnaya meditsina 2009; 4: 27–33.
9. Novikov V.V., Evsegneeva I.V., Karaulov A.V., Baryshnikov A.Yu. Soluble immune cell membrane antigen in socially important infections. Report 2. The study of their role in viral infections. Rossiyskiy bioterapevticheskiy zhurnal 2005; 4(3): 131–145.
10. Verbuggen L.A., Dumarey N., Van de Velde H. Soluble HLA–DR antigen levels in serum correlate with rheumatoid arthritis disease activity and the presence of disease — associated epitopes. Tissue Antigens 2000; 56(5): 436–440.
11. Stevenson F.K., George A.J., Walters M.T., Hamblin T.J. Analysis of soluble HLA class II antigenic material in patients with immunological diseases using monoclonal antibodies. J Immunol Methods 1986; 86(2): 187–190.
12. Korovushkina K.A. Sravnitel'naya otsenka biokhimicheskikh i immunologicheskikh pokazateley pri dobrokachestvennykh i zlokachestvennykh opukholyakh zhenskoy polovoy sfery. Avtoref. dis. … kand. biol. nauk [Comparative assessment of biochemical and immunological indices in benign and malignant tumors of female genital zone. Abstract of Dissertation for the degree of Candidate of Biological science]. Nizhny Novgorod; 2010.
13. Yarilin A.A. Osnovy immunologii [Basics of immunology]. Moscow: Meditsina; 1999; 608 p.
14. Golenkov A.K., Mitina T.A., Novikov V.V., et al. Clinical value of soluble adhesion (sCD50–ICAM-3), apoptosis (sCD95) and sHLA class I molecules in lymphoprofilerative diseases. Rossiyskiy bioterapevticheskiy zhurnal 2002, 1: 60–64.
15. Golovizin M.V. Intervention of tumor cells in T-lymphocyte maturation and selection processes as a neoplastic proliferation factor. Immunologiya 2001; 6: 4–10.
16. Perenkov A.D., Novikov D.V., Sakharnov N.A., Alyasova A.V., Utkin O.V., Baryshnikov A.Yu., Novikov V.V. Heterogeneous CD38 expression in tumor tissues of patients with colorectal cancer. Molecular Biology 2012; 46(5): 705–709.

Mamaeva М.Е., Shumilova S.V., Kazatskaya Zh.А., Khazov М.V., Novikov V.V., Alyasova А.V. The Content of Soluble HLA Class I and HLA-DR Molecules in Serum in Patients with Uterine Cervix and Body Pathology. Sovremennye tehnologii v medicine 2014; 6(2): 85