Today: Dec 6, 2024
RU / EN
Last update: Oct 30, 2024
Indices of Free Radical Oxidation in the Oral Fluid as Markers of Athletes’ Functional State

Indices of Free Radical Oxidation in the Oral Fluid as Markers of Athletes’ Functional State

Kontorshchikova K.N., Тikhomirova Y.R., Ovchinnikov А.N., Kolegova Т.I., Churkina N.N., Kuznetsova S.Y., Krylov V.N.
Key words: creatine kinase; lactate dehydrogenase; creatinine; lactate; glucose; free fatty acids; prooxidant-antioxidant homeostasis; oral fluid; physical exercise.
2017, volume 9, issue 3, page 82.

Full text

html pdf
2969
2122

The aim of the investigation was to study the efficiency of determining indices of free radical and peroxide processes in the oral fluid in highly qualified athletes under anaerobic interval exercise for the assessment of their functional state.

Materials and Methods. 23 highly qualified athletes at the age of 18.7±0.6 years participated in the study. The following methods were used to investigate homeostasis indices: induced biochemiluminescence, determination of primary and end lipid peroxidation product content according to a modified Volchegorsky method, enzymatic colorimetric method of measuring the concentration of creatinine, lactate, glucose and free fatty acids, enzymatic kinetic method of determining the quantitative activity of creatine kinase and lactate dehydrogenase in the oral fluid.

Results. Anaerobic interval exercise is estimated to initiate increase of lactate dehydrogenase activity and lactate concentration, reduction of all parameters of chemiluminescence reaction curve, and elevation of the content of trien conjugates and Schiff bases in the oral fluid of highly qualified athletes.

Conclusion. The study of free radical oxidation indices as well as the content of primary and end products of lipid peroxidation is an available, highly informative method of examining the functional state, adaptive mechanisms, and spare capacities of athletes at all stages of training and competitive processes.

  1. World Anti-Doping Agency. The World Anti-Doping Code. International Standard. Prohibited List 2017. URL: https://www.wada-ama.org/sites/default/files/resources/files/2016-09-29_-_wada_prohibited_list_2017_eng_final.pdf.
  2. Komarova L.G., Alekseeva O.P. Salivalogiya [Salivalogy]. Nizhny Novgorod: NizhGMA; 2006; 180 p.
  3. Gil’miyarova F.N., Radomskaya V.M., Ryskina E.A., et al. Analiticheskie podkhody k izucheniyu pokazateley metabolizma v rotovoy zhidkosti [Analytical approaches to the study of metabolism indices in the oral fluid]. Moscow: Izvestiya; 2006; 312 p.
  4. Guilhem G., Hanon C., Gendreau N., Bonneau D., Guével A., Chennaoui M. Salivary hormones response to preparation and pre-competitive training of world-class level athletes. Front Physiol 2015; 6: 333, https://doi.org/10.3389/fphys.2015.00333.
  5. Moreira A., Bacurau R.F., Napimoga M.H., Arruda A.F., Freitas C.G., Drago G., Aoki M.S. Salivary IL-21 and IgA responses to a competitive match in elite basketball players. Biol Sport 2013; 30(4): 243–247, https://doi.org/10.5604/20831862.1077548.
  6. Tékus E., Kaj M., Szabó E., Szénási N.L., Kerepesi I., Figler M., Gábriel R., Wilhelm M. Comparison of blood and saliva lactate level after maximum intensity exercise. Acta Biol Hung 2012; 63(Suppl 1): 89–98, https://doi.org/10.1556/abiol.63.2012.suppl.1.9.
  7. Damirchi A., Saati Zareei A., Sariri R. Salivary antioxidants of male athletes after aerobic exercise and garlic supplementation on: a randomized, double blind, placebo-controlled study. J Oral Biol Craniofac Res 2015; 5(3): 146–152, https://doi.org/10.1016/j.jobcr.2015.08.001.
  8. Sant’Anna M., Casimiro-Lopes G., Boaventura G., Marques S.T., Sorenson M.M., Simão R., Pinto V.S. Anaerobic exercise affects the saliva antioxidant/oxidant balance in high-performance pentathlon athletes. Human Movement 2016; 17(1): 50–55, https://doi.org/10.1515/humo-2016-0003.
  9. Kuz’mina E.I., Nelyubin A.S., Shchennikova M.K. Primenenie indutsirovannoy khemilyuminestsentsii dlya otsenki svobodnoradikal’nykh reaktsiy v biologicheskikh substratakh. V kn.: Biokhimiya i biofizika mikroorganizmov [The use of induced chemiluminescence to assess free radical reactions in biotic substrates. In: Biochemistry and biophysics of microorganisms]. Gorky; 1983; p. 179–183.
  10. Volchegorsky I.A., Nalimov A.G., Yarovinskiy B.G., Lifshits R.I. Comparison of various approaches to determination of lipid peroxidation products in heptane-isopropanol extracts of blood. Voprosy meditsinskoy khimii 1989; 35(1): 127–131.
  11. Mikhaylov S.S. Sportivnaya biokhimiya [Biochemistry for sport]. Moscow: Sovetskiy sport; 2006; 260 p.
  12. Gunina L.M. Oxidative stress and adaptation: metabolic aspects of physical activity impact. Nauka v olimpiyskom sporte 2013; 4: 19–25.
Kontorshchikova K.N., Тikhomirova Y.R., Ovchinnikov А.N., Kolegova Т.I., Churkina N.N., Kuznetsova S.Y., Krylov V.N. Indices of Free Radical Oxidation in the Oral Fluid as Markers of Athletes’ Functional State. Sovremennye tehnologii v medicine 2017; 9(3): 82, https://doi.org/10.17691/stm2017.9.3.11


Journal in Databases

pubmed_logo.jpg

web_of_science.jpg

scopus.jpg

crossref.jpg

ebsco.jpg

embase.jpg

ulrich.jpg

cyberleninka.jpg

e-library.jpg

lan.jpg

ajd.jpg

SCImago Journal & Country Rank