The Protective Role of N,N’-Dimethylthiourea and Its Effect on Hydrogen Peroxide Level of Hela Kyoto Cells under Cisplatin Action

The aim of the study is to sense the level of hydrogen peroxide (H₂O₂) in viable and apoptotic HeLa Kyoto cells under the action of cisplatin in presence of reactive oxygen species scavenger N,N’-dimethylthiourea (DMTU).

Materials and Methods. HeLa Kyoto cell line transfected with hydrogen peroxide sensor HyPer2 or pH sensor SypHer2 was incubated with cisplatin for 24 h with or without DMTU. The viability of the cells was determined by trypan blue staining technique and flow cytometry with apoptosis marker and vital dye. The response of the sensors was determined in viable cells and in cells in early apoptosis separately.

Results. A decrease in the percentage of viable cells upon exposure to cisplatin inhibited by the addition of DMTU to the incubation medium was shown. A significant increase in H₂O₂ level in HeLa Kyoto cells was observed upon drug exposure, while incubation with cisplatin and DMTU simultaneously resulted in a value close to the control one. The observed reaction was typical for both viable and apoptotic cells.

Conclusion. The protective role of DMTU and the involvement of H₂O₂ in tumor cells death under the cisplatin action was proved.

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Nerush A.S., Shchukina K.M., Orlova А.G. The Protective Role of N,N’-Dimethylthiourea and Its Effect on Hydrogen Peroxide Level of Hela Kyoto Cells under Cisplatin Action. Sovremennye tehnologii v medicine 2018; 10(3): 78, https://doi.org/10.17691/stm2018.10.3.9