Протекторная роль N,N’-диметилтиомочевины и ее влияние на внутриклеточный уровень пероксида водорода при воздействии цисплатина на клетки HeLa Kyoto

Цель исследования — оценка содержания пероксида водорода (Н₂О₂) в жизнеспособных и апоптотических клетках HeLa Kyoto при воздействии цисплатина в условиях применения ловушки активных форм кислорода — N,N’-диметилтиомочевины (ДМТМ).

Материалы и методы. Клетки линии HeLa Kyoto, трансфицированные сенсором пероксида водорода HyPer2 или сенсором рН SypHer2, подвергали инкубации с цисплатином в течение 24 ч в присутствии или в отсутствие ДМТМ. Жизнеспособность клеток определяли методом окрашивания трипановым синим и проточной цитофлюориметрии с маркером апоптоза и витальным красителем. Отклик сенсоров определяли отдельно в жизнеспособных клетках и в клетках в стадии раннего апоптоза.

Результаты. Установлено снижение процента жизнеспособных клеток при воздействии цисплатина, которое ингибируется добавлением ДМТМ в инкубационную среду. Выявлено значительное увеличение содержания пероксида водорода в исследуемой культуре при воздействии цисплатина, в то время как при одновременной инкубации с цисплатином и ДМТМ значение было близко к контрольному. Наблюдаемая реакция характерна как для жизнеспособных, так и для апоптозных клеток.

Заключение. Доказаны протекторная роль ДМТМ и участие Н₂О₂ в гибели опухолевых клеток при воздействии цисплатина.

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