Интегрон-зависимые механизмы резистентности к антибиотикам у штаммов Escherichia coli, выделенных из человека и животных в двух провинциях Ирана

Бактерия Escherichia coli (кишечная палочка) считается основным пищевым патогеном, широко распространенным среди людей и животных. Штаммы E. coli становятся все более устойчивыми к антибиотикам, частично благодаря генам, находящимся в составе интегронов.

Цель исследования — установить связь между наличием интегронов и резистентностью к антибиотикам у штаммов E. coli, выделенных у человека и животных в провинциях Альборз и Исфахан в Иране.

Материалы и методы. Собрано по 20 биологических образцов от крупного рогатого скота и овец в провинции Исфахан, а также от домашней птицы и людей — в провинции Альборз. Кишечную палочку выделяли с использованием стандартных биохимических и бактериологических методов. Чувствительность к антибиотикам определяли методом диффузии в агаре с использованием дисков по Кирби–Бауэру. Для амплификации генов классов 1 и 2 интегронов — Int1 и Int2 — использовали дуплексную полимеразную цепную реакцию.

Результаты. В общей сложности 33 из 80 изолятов (41,25%) содержали интегронные гены. Среди них у 25 изолятов (31,25%) обнаружены интегроны класса 1, у 8 изолятов (10,0%) — интегроны класса 2. Интегрон-положительные штаммы оказались резистентными более чем к 6 антибиотикам.

Заключение. Выявленное широкое распространение интегронов среди изолятов E. coli, выделенных в провинции Альборз, свидетельствует о необходимости проведения регулярного надзора и мониторинга устойчивости бактерий, выделенных у людей и животных в Иране, к противомикробным препаратам, включая молекулярный скрининг интегронов.

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Reza Ranjbar, Hamed Moradi, Naser Harzandi, Roohollah Kheiri, Faham Khamesipour. Integron-Associated Antibiotic Resistance Patterns in Escherichia coli Strains Isolated from Human and Animal Sources in Two Provinces of Iran. Sovremennye tehnologii v medicine 2019; 11(4): 64, https://doi.org/10.17691/stm2019.11.4.07